General Cell Lines and Cell Cultures
Lonza Walkersville Prim Cell Opt 4D-Nufe Y Kit
Electroporation-based methods have so far required cells to be in suspension for transfection However the 4D-Nucleofector Y Unit allows direct Nucleofection of cells in adherence Cells which typically grow in adherence in culture can be kept and transfected by Nucleofection in their physiological state The Primary Cell Optimization 4D-Nucleofector Y Kit is suited for rapid determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector Y Unit Using one 24-well Dipping Electrode Array and our pmaxGFP Vector as positive control our two Primary Cell Nucleofector Solutions for adherent Nucleofection (AD1 and AD2) are tested in combination with a pre-selected set of programs plus controls The remaining 24-well Dipping Electrode Array can be used for optional fine tuning or performing the first experiment with the selected conditions
ATCC Enterobacter hormaechei subsp. steigerwaltii; Strain: SC10435
Enterobacter hormaechei subsp. steigerwaltii strain SC10435 was isolated in 1963 from human blood culture in Toronto, Canada. This strain is resistant to penicillins, cephalosporins, aztreonam, and pipercillin-tazobactam and it is sensitive to carbapenems. The depositor states that this strain produces a Class C, AmpC-type beta-lactamase (P99 cephalosporinase).
ATCC Ralstonia pickettii
Ralstonia pickettii strain [ICPB 3981, K-288] was isolated from a patient who had undergone a tracheotomy. This culture is a whole-genome sequenced bacterial type strain.
Eurofins Discoverx PathHunter U2OS MC3R β-Arrestin Cell Line, 2 vials. (93-0984C3)
The PathHunter MC3R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. α-MSH) based activation of MC3R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC3R and EA-tagged β-Arrestin-2. Activation of MC3R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter LTB4R Total Internalization U2OS Cell Line, 2 vials. (93-0810C3)
The PathHunter LTB4R Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of LTB4R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged LTB4R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the LTB4R receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen D5 Dopamine Receptor Membrane Preparation, 1 vial. (HTS129M)
The ChemiScreen D5 Dopamine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of DRD5 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx PathHunter HEK 293 ADRA1D β-Arrestin Cell line, 2 vials. (93-0983C1)
The PathHunter ADRA1D cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1D GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA1D and EA-tagged β-Arrestin-2. Activation of ADRA1D stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Staphylococcus aureus; Quantitative DNA
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load and for other molecular biology applications.
Eurofins Discoverx ChemiBrite EP1 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS099L)
The ChemiBrite EP1 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER1, a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER1, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay PK2 Prokinecticin Receptor Frozen Cells, 2 Vials of Cells. (HTS182RTA)
The Ready-to-Assay PK2 Prokinecticin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prokinectin, EG-VEGF) based activation of PROKR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PROKR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR2, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.